DDAH1 Antibody - C-terminal region (OAAB01665)

Catalog No: OAAB01665

Size:400ul

Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of DDAH1 Antibody (C-term) (Cat. #OAAB01665) in mouse kidney tissue lysates (35ug/lane). DDAH1 (arrow) was detected using the purified Pab.

DDAH1 Antibody (C-term) (Cat. #OAAB01665) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of DDAH1 Antibody (C-term)(Cat#OAAB01665) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC, IF, IHC-P, WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DDAH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human DDAH1.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IHC-P: 1:50~100
  WB: 1:1000
  FC: 1:10~50
  IF: 1:10~50
• Other Applications Image 1 Data: DDAH1 Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: DDAH1
• Alias Symbols: DDAH, DDAHI, DDAH-1, HEL-S-16
• NCBI Gene Id: 23576
• Protein Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
• Uniprot ID: O94760
• Protein Accession #: NP_001127917.1
• Molecular Weight: 31122 Da