DCX ELISA Kit (Mouse) (OKDD00730)

Catalog No: OKDD00730 (Formerly GWB-KBBXS2)

Size:96 Wells

Mouse DCX ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology DCX ELISA Kit (Mouse) (OKDD00730) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for DCX has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DCX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DCX captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

  Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

  Mean Intra-Assay: CV<10%

  Mean Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-DCX Microplate: 96 Wells (12 x 8 Well Strips)
  • DCX Lyophilized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X DCX Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell lysates
• Sensitivity: <0.051 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of DCX. No significant cross-reactivity or interference between DCX and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Dcx
• Gene Full Name: doublecortin
• Alias Symbols: Dbct;doublecortex;doublin;lissencephalin-X;lissencephaly, X-linked (doublecortin);lis-X;neuronal migration protein doublecortin.
• NCBI Gene Id: 13193
• Protein Name: Neuronal migration protein doublecortin
• Description of Target: Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May participate along with PAFAH1B1/LIS-1 in a distinct overlapping signaling pathway that promotes neuronal migration.
• Uniprot ID: O88809
• Protein Accession #: NP_001103692.1
• Nucleotide Accession #: NM_001110222.1
• Protein Size (# AA): 366
• Molecular Weight: 41 kDa