DCTN1 Antibody (OAEB02346)

Catalog No: OAEB02346 (Formerly GWB-D62D50)

Size:100UG

OAEB02346 staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OAEB02346 (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: DCTN1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 92%
• Peptide Sequence: C-QEQLHQLHSRLIS
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:128000.
  Western Blot: Approx 150kDa band observed in cell lline A549 and in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2ug/ml.
  Immunohistochemistry: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells.
  In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4ug/ml.
• Reference: * Bupha-Intr T, Haizlip KM, Janssen PM.; Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro.; Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H806-14.; Pubmed 19136602
  * Magnani E, Fan J, Gasparini L, Golding M, Williams M, Schiavo G, Goedert M, Amos LA, Spillantini MG.; Interaction of tau protein with the dynactin complex.; EMBO J. 2007 Oct 31;26(21):4546-54.; Pubmed 17932487
  * Holzbaur EL, Tokito MK.; Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization.; Genomics. 1996 Feb 1;31(3):398-9. No abstract available.; Pubmed 8838327

Target Info

• Gene Symbol: Dctn1|DCTN1
• Gene Full Name: dynactin 1|dynactin subunit 1
• Alias Symbols: 150 kDa dynein-associated polypeptide;AL022633;DAP-150;DP-150;dynactin 1 (p150, glued homolog, Drosophila);dynactin 1, retrograde axonal transport;dynactin subunit 1;Glue;Glued;P135;p15;p150;p150;p150-glued.
• NCBI Gene Id: 13191|1639
• Protein Name: Dynactin subunit 1
• Uniprot ID: Q14203
• Protein Accession #: NP_004073.2
• Molecular Weight: 142; 127; 139; 127, 136, 140