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DCTN1 Antibody (OAEB02346)

100 ug
$299.00
In Stock
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Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clonality:
Polyclonal
Host:
Goat
Application:
WB, IHC
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Replacement Item:
This antibody may replace item sc-135890 from Santa Cruz Biotechnology.
Immunogen:
This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).
Predicted Homology Based on Immunogen Sequence:
Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 92%
Complete computational species homology data:
DCTN1 Antibody (OAEB02346)
Peptide Sequence:
C-QEQLHQLHSRLIS
Datasheets/Manuals:
Printable datasheet for anti-DCTN1 antibody - OAEB02346
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot: Approx 150kDa band observed in cell lline A549 and in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2ug/ml.
Immunohistochemistry: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells.
In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4ug/ml.
Target Reference:
* Bupha-Intr T, Haizlip KM, Janssen PM.; Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro.; Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H806-14.; Pubmed 19136602
* Magnani E, Fan J, Gasparini L, Golding M, Williams M, Schiavo G, Goedert M, Amos LA, Spillantini MG.; Interaction of tau protein with the dynactin complex.; EMBO J. 2007 Oct 31;26(21):4546-54.; Pubmed 17932487
* Holzbaur EL, Tokito MK.; Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization.; Genomics. 1996 Feb 1;31(3):398-9. No abstract available.; Pubmed 8838327
Gene Symbol:
DCTN1
Alias Symbols:
DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin
NCBI Gene Id:
1639
Protein Name:
Dynactin subunit 1
Swissprot Id:
Q14203
Protein Accession #:
NP_004073.2
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express DCTN1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express DCTN1.

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