DCTN1 Antibody - N-terminal region (OAAB21155)

Catalog No: OAAB21155

Size:100ul

Western blot detection of Dynactin 1 in K562, MCF7, 293T and Hela cell lysates using Dynactin 1 mouse mAb (OAAB21155) (1:500 diluted). Predicted band size: 150KDa. Observed band size: 150KDa.

Immunoprecipitation analysis of Hela cell lysates using Dynactin 1 mouse mAb (OAAB21155).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Mouse
• Application: WB, IP
• Additional Information: Function: Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein- dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:500
  IP: 1:500
• Reference: Collin G.B.,et al.Genomics 53:359-364(1998).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Hillier L.W.,et al.Nature 434:724-731(2005).
  Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
  Holzbaur E.L.F.,et al.Genomics 31:398-399(1996).
  

Target Info

• Gene Symbol: DCTN1
• Gene Full Name: dynactin subunit 1
• Alias Symbols: P135, DP-150, DAP-150
• NCBI Gene Id: 1639
• Description of Target: This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminal. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).
• Uniprot ID: Q14203
• Protein Accession #: NP_001128512.1
• Nucleotide Accession #: NM_001135040.2
• Molecular Weight: 142 kDa