Size:96 Wells
$610.00
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SKU
OKCA01193
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DCST1 ELISA Kit (Human) (OKCA01193)

Catalog#: OKCA01193
Domestic: within 1-2 weeks delivery International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3.5 Hours
Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat8581-90
1:29895-102
1:49285-96
1:810297-106
Kit Principle Aviva Systems Biology DCST1 ELISA Kit (Human) (OKCA01193) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DCST1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DCST1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DCST1 captured in the well.
Kit Range 25 - 1,600 pg/mL
Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)9187-96
EDTA Plasma (n=4)10398-105
Kit Reproducibility Intra-assay Precision: 3 samples with known low, middle and high level OKCA01193 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01193 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Component
ComponentAmount
DCST1 Microplate96 Wells (12 x 8 Well strips)
DCST1 Lyophilized Standard2 x 1.6 ng
100X Biotinylated DCST1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity 6.25 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info Assay Methodology: Quantitative Sanadwich ELISA
Gene Symbol DCST1
Official Gene Full Name DC-STAMP domain containing 1
Alias Symbols FLJ32785, RP11-307C12.10
NCBI Gene Id 149095
Protein Name DC-STAMP domain-containing protein 1
Description of Target This gene encodes a protein with a domain similar to one found in dendritic cells (PMID:11169400) which play a key role in antigen processing and display for immune responses. Multiple transcript variants encoding different isoforms have been found for this gene.
Swissprot Id Q5T197
Protein Accession # NP_001137159.1
Nucleotide Accession # NM_001143687.2
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express DCST1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express DCST1.
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