DCN ELISA Kit (Human) (OKCD08009)

Catalog No: OKCD08009

Size:96WELLS

DCN ELISA Kit (Human) (OKCD08009) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Decorin (DCN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Decorin (DCN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Decorin (DCN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Decorin (DCN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.25-80ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Decorin (DCN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Decorin (DCN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • DCN Microplate: 96 Wells (12 x 8 Well strips)
  • DCN Lyophilized Standard: 2
  • 100X DCN HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.54ng/m

Target Info

• Gene Symbol: DCN
• Gene Full Name: decorin
• Alias Symbols: bone proteoglycan II;CSCD;decorin;dermatan sulphate proteoglycans II;DSPG2;PG40;PGII;PGS2;PG-S2;proteoglycan core protein;SLRR1B;small leucine-rich protein 1B.
• NCBI Gene Id: 1634
• Protein Name: Decorin
• Description of Target: May affect the rate of fibrils formation.
• Uniprot ID: P07585
• Protein Accession #: NP_001911.1
• Nucleotide Accession #: NM_001920.4