DCLRE1C Antibody - N-terminal region (OAAB06337)

Catalog No: OAAB06337

Size:400UL

Western blot analysis of DCLRE1C Antibody (N-term) in mouse heart tissue lysates (35ug/lane). DCLRE1C (arrow) was detected using the purified Pab.

DCLRE1C Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCLRE1C Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DCLRE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human DCLRE1C.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:2000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: DCLRE1C
• Alias Symbols: A-SCID, DCLREC1C, DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae), DNA cross-link repair 1C protein, Protein A-SCID, protein artemis, RS-SCID, SCIDA, severe combined immunodeficiency, type a (Athabascan), SNM1 homolog C, SNM1-like protein, SNM1C.
• NCBI Gene Id: 64421
• Protein Name: Protein artemis|protein artemis isoform a [Homo sapiens]|protein artemis isoform b [Homo sapiens]|protein artemis isoform c [Homo sapiens]
• Description of Target: Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.
• Uniprot ID: Q96SD1
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001029027.1,NP_001029029.1,NP_001029030.1,NP_071932.2
• Molecular Weight: 78436