Catalog No: OKCD08785
Size:96WELLS
Price: $800.00
SKU
OKCD08785
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Datasheets/ManualsPrintable datasheet for DCLK1 ELISA Kit (Human) (OKCD08785)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Doublecortin Like Kinase 1 (DCLK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Doublecortin Like Kinase 1 (DCLK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Doublecortin Like Kinase 1 (DCLK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Doublecortin Like Kinase 1 (DCLK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Doublecortin Like Kinase 1 (DCLK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Doublecortin Like Kinase 1 (DCLK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
DCLK1 Microplate96 Wells (12 x 8 Well strips)
DCLK1 Lyophilized Standard2
100X DCLK1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.054ng/mL
Gene SymbolDCLK1
Gene Full Namedoublecortin like kinase 1
Alias SymbolsCL1;CLICK1;DCAMKL1;DCDC3A;DCLK;doublecortin domain-containing protein 3A;doublecortin-like and CAM kinase-like 1;Doublecortin-like kinase 1;serine/threonine-protein kinase DCLK1.
NCBI Gene Id9201
Protein NameSerine/threonine-protein kinase DCLK1
Description of TargetProbable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Uniprot IDO15075
Protein Accession #NP_001182344.1
Nucleotide Accession #NM_001195415.1
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