DCK Antibody (OAGA00587)

Catalog No: OAGA00587

Size:100 ul

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
OAGA00587 diluted at 1:1000

DCK antibody detects DCK protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
12 % SDS-PAGE
DCK antibody (OAGA00587) dilution: 1:1000

Sample(30 ug whole cell lysate)
A:Raji
12% SDS PAGE
OAGA00587 diluted at 1:1000

DCK antibody detects DCK protein at cytosol and nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: DCK antibody (OAGA00587) dilution: 1:250.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Deoxycytidine kinase(OAGA00587) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq]
  Positive Controls: Raji, mouse brain, PC-12, Rat2
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 260 of DCK (Uniprot ID#P27707)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: DCK
• Gene Full Name: Deoxycytidine kinase
• Alias Symbols: MGC117410 antibody, MGC138632 antibody, DCK antibody, deoxycytidine kinase antibody, deoxynucleoside kinase antibody
• NCBI Gene Id: 1633
• Protein Name: Deoxycytidine kinase
• Uniprot ID: P27707
• Protein Accession #: NP_000779.1
• Molecular Weight: 31 kDa