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Catalog No: OCOA12717
Size:20UG
Price: $275.00
SKU
OCOA12717
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DCC Protein Lysate (OCOA12717)

Datasheets/ManualsPrintable datasheet for DCC Protein Lysate (OCOA12717)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionDCC HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolDCC
Gene Full NameDCC netrin 1 receptor
Alias Symbolscolorectal cancer suppressor;CRC18;CRCR1;deleted in colorectal carcinoma;HGPPS2;IGDCC1;immunoglobulin superfamily DCC subclass member 1;MRMV1;netrin receptor DCC;NTN1R1;putative colorectal tumor suppressor;tumor suppressor protein DCC.
NCBI Gene Id1630
Protein NameNM_005215, NP_005206, P43146
Description of TargetHomo sapiens DCC netrin 1 receptor (DCC), mRNA, Netrin receptor DCC, netrin receptor DCC [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P43146
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_005206
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_005215
Molecular Weight158.3 kDa
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