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Catalog No: OCOA04576
Size:20UG
Price: $275.00
SKU
OCOA04576
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DCAF11 Protein Lysate (OCOA04576)

Datasheets/ManualsPrintable datasheet for DCAF11 Protein Lysate (OCOA04576)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionDCAF11 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolDCAF11
Gene Full NameDDB1 and CUL4 associated factor 11
Alias SymbolsDDB1- and CUL4-associated factor 11;GL014;PRO2389;WD repeat domain 23;WD repeat-containing protein 23;WDR23.
NCBI Gene Id80344
Protein NameB3KSW2, NM_181357, NP_852002, Q8TEB1
Description of TargetcDNA FLJ37159 fis, clone BRACE2026294, moderately similar to WD repeat protein 23, DDB1- and CUL4-associated factor 11, DDB1- and CUL4-associated factor 11 isoform 2 [Homo sapiens], Homo sapiens DDB1 and CUL4 associated factor 11 (DCAF11), transcript variant 2, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q8TEB1
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_852002
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_181357
Molecular Weight58.7 kDa
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