Catalog No: OCOA14377
Size:20UG
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Datasheets/Manuals | Printable datasheet for DBT Protein Lysate (OCOA14377) |
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Predicted Species Reactivity | Homo sapiens |
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Product Format | Lyophilized |
Host | HEK293T |
Additional Information | Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
:: | Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reconstitution and Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes |
Tag | C-MYC/DDK |
Description | DBT HEK293T cell transient overexpression lysate (as WB positive control) |
Gene Symbol | DBT |
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Gene Full Name | dihydrolipoamide branched chain transacylase E2 |
Alias Symbols | 52 kDa mitochondrial autoantigen of primary biliary cirrhosis;BCATE2;BCKAD E2 subunit;BCKADE2;BCKAD-E2;BCKDH-E2;BCOADC-E2;branched chain 2-oxo-acid dehydrogenase complex component E2;branched chain acyltransferase, E2 component;branched-chain alpha-keto acid dehydrogenase complex component E2;dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex;Dihydrolipoamide branched chain transacylase;dihydrolipoyl transacylase;dihydrolipoyllysine-residue (2-methylpropanoyl)transferase;E2;E2 component of branched chain alpha-keto acid dehydrogenase complex;E2B;lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial;lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex;mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b). |
NCBI Gene Id | 1629 |
Protein Name | NM_001918, NP_001909, P11182 |
Description of Target | Homo sapiens dihydrolipoamide branched chain transacylase E2 (DBT), mRNA, Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial, lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial precursor [Homo sapiens] |
Uniprot ID | https://www.uniprot.org/uniprot/P11182 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_001909 |
Nucleotide Accession # | https://www.ncbi.nlm.nih.gov/nuccore/NM_001918 |
Molecular Weight | 53.5 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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