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Catalog No: OKEH07969
Size:96T
Price: $800.00
SKU
OKEH07969
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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DBT ELISA Kit (Bovine) (OKEH07969)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology DBT ELISA Kit (Bovine) (OKEH07969) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DBT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DBT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DBT captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=6.6% (n = 20)
Mean Inter-assay CV%: <=6.7% (n = 20)
ELISA Kit Component
ComponentAmount
DBT Microplate96 Wells (12 x 8 Well strips)
DBT Lyophilized Standard2
100X Biotinylated DBT Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.
::Subcellular Location: Mitochondrion matrix
::Unigene: Bt.107201
SMR: P11181
String: 9913.ENSBTAP00000008292
::KEGG: bta:280759
Pfam: PF00198
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Bovine Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDBT
Gene Full Namedihydrolipoamide branched chain transacylase E2
Alias SymbolsBCKAD E2 subunit;BCKADE2;BCKAD-E2;branched-chain alpha-keto acid dehydrogenase complex component E2;component of branched chain keto acid dehydrogenase complex;dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex;Dihydrolipoamide branched chain transacylase;dihydrolipoyllysine-residue (2-methylpropanoyl)transferase;E2;E2b;lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial.
NCBI Gene Id280759
Protein NameP11181
Description of TargetLipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P11181
Protein Accession #NP_776330.1
Nucleotide Accession #NM_173905.2
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