DBT Antibody (OAGA04317)

Catalog No: OAGA04317

Size:100 ul

DBT antibody detects DBT protein by western blot analysis.
A. 50 ug mouse liver lysate/extract
10% SDS-PAGE
DBT antibody dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

DBT antibody detects DBT protein by western blot analysis.
A. 50 ug rat liver lysate/extract
10% SDS-PAGE
DBT antibody dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: H1299
B: HepG2
10% SDS PAGE
diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

DBT antibody detects DBT protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
DBT antibody diluted at 1:500.

Immunofluorescence analysis of methanol-fixed A431, using DBT antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DBT. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.33 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: DBT
• Gene Full Name: dihydrolipoamide branched chain transacylase E2
• Alias Symbols: E2, E2B, BCATE2, BCKADE2, BCKAD-E2, BCKDH-E2, BCOADC-E2
• NCBI Gene Id: 1629
• Protein Name: lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
• Description of Target: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
• Uniprot ID: P11182
• Protein Accession #: NP_001909.3
• Nucleotide Accession #: NM_001918.3