Dbp ELISA Kit (Rat) (OKCD01991)

Catalog No: OKCD01991 (Formerly GWB-KBBLC6)

Size:96WELLS

Rat Dbp ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D Site Of Albumin Promoter Binding Protein (DBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D Site Of Albumin Promoter Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D Site Of Albumin Promoter Binding Protein (DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of D Site Of Albumin Promoter Binding Protein (DBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D Site Of Albumin Promoter Binding Protein (DBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D Site Of Albumin Promoter Binding Protein (DBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Dbp Microplate: 96 Wells (12 x 8 Well Strips)
  • Dbp Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Dbp Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.105 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of D Site Of Albumin Promoter Binding Protein (DBP). No significant cross-reactivity or interference between D Site Of Albumin Promoter Binding Protein (DBP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DBP
• Gene Full Name: D-box binding PAR bZIP transcription factor
• Alias Symbols: Albumin D box-binding protein, Albumin D-element-binding protein, D site albumin promoter-binding protein 1, D site-binding protein
• NCBI Gene Id: 24309
• Protein Name: D site-binding protein
• Description of Target: This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation.
• Uniprot ID: P16443
• Protein Accession #: NP_036675.1
• Nucleotide Accession #: NM_012543.3
• Protein Size (# AA): 325