Catalog No: OKCD00402 (Formerly GWB-KBBIZ7)
Size:96WELLS
Price: $700.00
SKU
OKCD00402
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)81-101%98-105%90-99%79-99%
EDTA plasma(n=5)81-98%94-102%87-102%91-98%
heparin plasma(n=5)96-104%97-105%79-91%79-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diazepam Binding Inhibitor (DBI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diazepam Binding Inhibitor (DBI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diazepam Binding Inhibitor (DBI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Diazepam Binding Inhibitor (DBI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)86-10197
EDTA plasma(n=5)90-10497
heparin plasma(n=5)81-9987
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diazepam Binding Inhibitor (DBI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diazepam Binding Inhibitor (DBI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Dbi Microplate96 Wells (12 x 8 Well Strips)
Dbi Lyophilized Standard2 x 10 ng
100X Biotinylated Dbi Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity< 2.67 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Diazepam Binding Inhibitor (DBI). No significant cross-reactivity or interference between Diazepam Binding Inhibitor (DBI) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDbi
Gene Full Namediazepam binding inhibitor
Alias SymbolsAc;ACBD1;Acbp;acyl-CoA-binding protein;diazepam binding inhibitor, splice form 1b;Diazepam-binding inhibitor;E;endoz;endozepine;EP.
NCBI Gene Id13167
Protein NameAcyl-CoA-binding protein
Description of TargetBinds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
Uniprot IDP31786
Protein Accession #NP_031856
Nucleotide Accession #NM_007830.4
Protein Size (# AA)87
Write Your Own Review
You're reviewing:DBI ELISA Kit (Mouse) (OKCD00402)
Your Rating
We found other products you might like!