Size:96 Wells
$549.00
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SKU
OKEH05500
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DBH ELISA Kit (Mouse) (OKEH05500)

Catalog#: OKEH05500
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species ReactivityMouse
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~3 Hours
Kit PrincipleAviva Systems Biology Dbh ELISA Kit (Mouse) (OKEH05500) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dbh has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dbh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dbh captured in the well.
Kit Range0.78 - 50ng/mL
Kit RecoveryMean recovery when spiking into Serum and Plasma = 101.8%
Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <8.3%, n = 20
Mean Inter-Assay Precision - <10.0%, n = 20
Kit Component
ComponentAmount
DBH Microplate96 Wells (12 x 8 Well strips)
DBH Lyophilized Standard2
100X Biotinylated DBH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.39 ng/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Mouse Dopamine beta-hydroxylase
PublicationsPark G., Jung Y.S., Park M.K., Yang CH, Kim, Y.U. Melatonin inhibits attention-deficit/hyperactivity disorder caused by atopic dermatitis-induced psychological stress in an NC/Nga atopic-like mouse model. Sci Rep. 2018 Oct 8;8(1):14981. 30297827

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDBH
Official Gene Full NameDopamine beta hydroxylase
Alias SymbolsDopamine beta-hydroxylase, Dopamine beta-monooxygenase
NCBI Gene Id13166
Protein NameDopamine beta-hydroxylase
Description of TargetConversion of dopamine to noradrenaline.
Swissprot IdQ64237
Protein Accession #NP_620392.2
Nucleotide Accession #NM_138942.3
Protein Size (# AA)622
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express DBH.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express DBH.
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