Size:96 Wells
$549.00
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SKU
OKEH05500
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DBH ELISA Kit (Mouse) (OKEH05500)

Catalog#: OKEH05500
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~3 Hours
Kit Principle Aviva Systems Biology Dbh ELISA Kit (Mouse) (OKEH05500) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dbh has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dbh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dbh captured in the well.
Kit Range 0.78 - 50ng/mL
Kit Recovery Mean recovery when spiking into Serum and Plasma = 101.8%
Kit Reproducibility Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <8.3%, n = 20
Mean Inter-Assay Precision - <10.0%, n = 20
Kit Component
ComponentAmount
DBH Microplate96 Wells (12 x 8 Well strips)
DBH Lyophilized Standard2
100X Biotinylated DBH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma and other biological fluids
Sensitivity 0.39 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse Dopamine beta-hydroxylase
Publications Park G., Jung Y.S., Park M.K., Yang CH, Kim, Y.U. Melatonin inhibits attention-deficit/hyperactivity disorder caused by atopic dermatitis-induced psychological stress in an NC/Nga atopic-like mouse model. Sci Rep. 2018 Oct 8;8(1):14981. 30297827

Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol DBH
Official Gene Full Name Dopamine beta hydroxylase
Alias Symbols Dopamine beta-hydroxylase, Dopamine beta-monooxygenase
NCBI Gene Id 13166
Protein Name Dopamine beta-hydroxylase
Description of Target Conversion of dopamine to noradrenaline.
Swissprot Id Q64237
Protein Accession # NP_620392.2
Nucleotide Accession # NM_138942.3
Protein Size (# AA) 622
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express DBH.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express DBH.
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