Catalog No: OAEE00384
Size:100UG
Price: $209.00
SKU
OAEE00384
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for DAXX Antibody (OAEE00384) (OAEE00384)
Product Info
Predicted Species ReactivityHuman
Product FormatPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
ClonalityMonoclonal
CloneDAXX-03
IsotypeIgG1
HostMouse
ApplicationImmunocytochemistry|Immunoprecipitation|Western blot
Reconstitution and Storage2°C to 8°C
ImmunogenHis6-tagged protein corresponding to amino acids 580-740 of human Daxx.
PurificationPurified
Concentration1 mg/ml
SpecificityThe antibody DAXX-03 reacts with C-terminal part of Daxx, a 110 kDa intracellular protein.
Purity> 95% (by SDS-PAGE)
Application InfoWB 2 ug/ml
Storage BufferPhosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
ReferenceSimova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36.
Jung YS, Kim HY, Kim J, Lee MG, Pouysségur J, Kim E: Physical Interactions and Functional Coupling between Daxx and Sodium Hydrogen Exchanger 1 in Ischemic Cell Death. J Biol Chem. 2008 Jan 11;283(2):1018-25.
Shih HM, Chang CC, Kuo HY, Lin DY: Daxx mediates SUMO-dependent transcriptional control and subnuclearcompartmentalization. Biochem Soc Trans. 2007 Dec;35(Pt 6):1397-400.
Li Q, Wang X, Wu X, Rui Y, Liu W, Wang J, Wang X, Liou YC, Ye Z, Lin SC: Daxx cooperates with the Axin/HIPK2/p53 complex to induce cell death. Cancer Res. 2007 Jan 1;67(1):66-74.
Croxton R, Puto LA, de Belle I, Thomas M, Torii S, Hanaii F, Cuddy M, Reed JC. Daxx represses expression of a subset of antiapoptotic genes regulated by nuclearfactor-kappaB. Cancer Res. 2006 Sep 15;66(18):9026-35.
Muromoto R, Ishida M, Sugiyama K, Sekine Y, Oritani K, Shimoda K, Matsuda T: Sumoylation of Daxx regulates IFN-induced growth suppression of B lymphocytes andthe hormone receptor-mediated transactivation. J Immunol. 2006 Jul 15;177(2):1160-70.
Gene SymbolDAXX
Gene Full Namedeath domain associated protein
Alias SymbolsBING2;CENP-C binding protein;DAP6;Daxx;death domain-associated protein 6;death-associated protein 6;EAP1;ETS1-associated protein 1;fas death domain-associated protein;Fas-binding protein;SMIM40.
NCBI Gene Id1616
Protein NameDeath domain-associated protein 6
Description of TargetTranscription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activation of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3.3. Acts as targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upon neuronal activation associates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML-NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3.3 into chromatin. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes; the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3.3 and H4 and decreases binding of CTCF to chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction activity towards human cytomegalovirus (HCMV). Plays a role as a positive regulator of the heat shock transcription factor HSF1 activity during the stress protein response (PubMed:15016915).
Uniprot IDQ9UER7
Protein Accession #NP_001135441.1; NP_001241646.1; NP_001341.1
Nucleotide Accession #NM_001141969.1; NM_001254717.1; NM_001350.4
Molecular Weight110 kDa
  1. What is the species homology for "DAXX Antibody (OAEE00384)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".

  2. How long will it take to receive "DAXX Antibody (OAEE00384)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "DAXX Antibody (OAEE00384)" provided in?

    This item is provided in "Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "DAXX Antibody (OAEE00384)"?

    This target may also be called "BING2;CENP-C binding protein;DAP6;Daxx;death domain-associated protein 6;death-associated protein 6;EAP1;ETS1-associated protein 1;fas death domain-associated protein;Fas-binding protein;SMIM40." in publications.

  5. What is the shipping cost for "DAXX Antibody (OAEE00384)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "DAXX Antibody (OAEE00384)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "DAXX Antibody (OAEE00384)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "110 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "DAXX Antibody (OAEE00384)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "DAXX"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "DAXX"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "DAXX"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "DAXX"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "DAXX"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "DAXX"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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