Catalog No: OKCD01992 (Formerly GWB-KBBDW5)
Size:96WELLS
Price: $675.00
SKU
OKCD01992
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for DAPP1 ELISA kit (Human) |
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Predicted Species Reactivity | Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colormetric|Regular detection | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides (DAPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides (DAPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides (DAPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides (DAPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides (DAPP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides (DAPP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C, -20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.055 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides (DAPP1). No significant cross-reactivity or interference between Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides (DAPP1) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | DAPP1 |
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Gene Full Name | Dual adaptor of phosphotyrosine and 3-phosphoinositides 1 |
Alias Symbols | BAM32 |
NCBI Gene Id | 27071 |
Protein Name | Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide |
Description of Target | May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K. |
Uniprot ID | Q9UN19 |
Protein Accession # | NP_001293080.1 |
Nucleotide Accession # | NM_001306151.1 |
Protein Size (# AA) | 280 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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