Catalog No: OKCD08130
Size:96WELLS
Price: $825.00
SKU
OKCD08130
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Datasheets/ManualsPrintable datasheet for DAPK1 ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Death Associated Protein Kinase 1 (DAPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Death Associated Protein Kinase 1 (DAPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Death Associated Protein Kinase 1 (DAPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Death Associated Protein Kinase 1 (DAPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Death Associated Protein Kinase 1 (DAPK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Death Associated Protein Kinase 1 (DAPK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
DAPK1 Microplate96 Wells (12 x 8 Well strips)
DAPK1 Lyophilized Standard2
100X DAPK1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.051ng/mL
Gene SymbolDAPK1
Gene Full Namedeath associated protein kinase 1
Alias SymbolsDAP-, D13Ucl, D13Ucla1, DAP-Kinase
NCBI Gene Id69635
Protein Namedeath-associated protein kinase 1
Description of TargetPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Uniprot IDQ80YE7
Protein Accession #NP_001272846.1
Nucleotide Accession #NM_001285917.1
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