DAOA Chemi-Luminescent ELISA Kit (Human) (OKCD03410)

Catalog No: OKCD03410

Size:96WELLS

Human DAOA ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to D-amino Acid Oxidase Activator (DAOA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to D-amino Acid Oxidase Activator (DAOA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the D-amino Acid Oxidase Activator (DAOA) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-amino Acid Oxidase Activator (DAOA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-amino Acid Oxidase Activator (DAOA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • DAOA Microplate: 96 Wells (12 x 8 Well strips)
  • DAOA Lyophilized Standard: 2
  • 100X DAOA Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DAOA
• Gene Full Name: D-amino acid oxidase activator
• Alias Symbols: D-amino acid oxidase activator;LG72;Protein G72;schizophrenia- and bipolar disorder-associated protein G72;SG72.
• NCBI Gene Id: 267012
• Protein Name: D-amino acid oxidase activator
• Description of Target: Seems to activate D-amino acid oxidase.
• Uniprot ID: P59103
• Protein Accession #: NP_001155284
• Nucleotide Accession #: NM_001161812