DAG1 Antibody (OAGA02461)

Catalog No: OAGA02461

Size:100 ul

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
OAGA02461 diluted at 1:500

alpha Dystroglycan antibody detects DAG1 protein at cytoplasm on colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
alpha Dystroglycan antibody (OAGA02461) dilution: 1:500.

alpha Dystroglycan antibody detects alpha Dystroglycan protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Dystroglycan protein stained by alpha Dystroglycan antibody (OAGA02461) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.
Blue: Hoechst 33342 staining.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using alpha Dystroglycan (OAGA02461) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Dystroglycan. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.66mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: DAG1
• Gene Full Name: dystroglycan 1 (dystrophin-associated glycoprotein 1)
• Alias Symbols: A3a, DAG, AGRNR, 156DAG, MDDGA9, MDDGC7, MDDGC9, LGMDR16
• NCBI Gene Id: 1605
• Description of Target: Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.
• Uniprot ID: Q14118