DAG1 Antibody - C-terminal region (OAGA07130)

Catalog No: OAGA07130

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
B: MCF-7
10% SDS PAGE
diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

beta dystroglycan antibody [C2C3-2], C-term detects beta dystroglycan protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
beta dystroglycan antibody [C2C3-2], C-term dilution: 1:250.

beta dystroglycan antibody [C2C3-2], C-term detects beta dystroglycan protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta dystroglycan protein stained by beta dystroglycan antibody [C2C3-2], C-term diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human beta dystroglycan. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: DAG1
• Gene Full Name: dystroglycan 1
• Alias Symbols: A3a, DAG, AGRNR, 156DAG, MDDGA9, MDDGC7, MDDGC9, LGMDR16
• NCBI Gene Id: 1605
• Protein Name: dystroglycan
• Description of Target: This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein.
• Uniprot ID: Q14118
• Protein Accession #: NP_001159400.2
• Nucleotide Accession #: NM_001165928.3