DAG1 Antibody - C - terminal region (OAAB10401)

Catalog No: OAAB10401

Size:400ul

DAG1 Antibody (C-term) (Cat. #OAAB10401) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DAG1 antibody detected the DAG1 protein (arrow).

DAG1 Antibody (C-term) (OAAB10401)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DAG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Pig, Rabbit
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, ELISA
• Additional Information: Cellular Location: Alpha-dystroglycan: Secreted, extracellular space.
  Tissue Location Expressed in a variety of fetal and adult tissues. In epidermal tissue, located to the basement membrane Also expressed in keratinocytes and fibroblasts.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 718-747 amino acids from the C-terminal region of human DAG1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.5 mg/ml
• Intended Use: DAG1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: DAG1
• Alias Symbols: A3a, DAG, AGRNR, 156DAG, MDDGA9, MDDGC7, MDDGC9, LGMDR16
• NCBI Gene Id: 1605
• Protein Name: Dystroglycan
• Description of Target: Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.
  
• Uniprot ID: Q14118
• Protein Accession #: NP_001159400.2
• Molecular Weight: 97 kDa