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Search Antibody, Protein, and ELISA Kit Solutions

DAB Chromogenic Kit (Yellow) (OKBB00046)

  • Catalog#: OKBB00046
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Application:
ELISA
Kit Component:
1. Chromogenic reagent A: DAB concentrated solution, 3ml. (20×)
2. Chromogenic reagent B: H2O2 concentrated solution, 3 ml. (20×)
3. Chromogenic reagent C: TBS concentrated buffer, 3ml. (20×)
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets/Manuals:
Printable datasheet for OKBB00046
Reconstitution and Storage:
Store at -20C for one year
Preparation:
1. After the peroxidase detection, wash with TBS or PBS 4 times and each time for 5 minutes.
2. Add one drop (about 50ul) of concentrated chromogenic reagent A, B and C respectively into 1ml of distilled water and mix evenly. Add this working solution to the sample and colorate in dark for 10-30 minutes. If no background appearing, keep coloration until satisfied.
3. Wash fully with distilled water to stop reaction. Counterstain with hematoxylin for about 1 minutes if necessary. Wash with water.
4. Seal slide with balsam neutral and observe with microscope.
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Notes: Please prepare the chromogenic reagent A, B and C working solution according to Step 2 of "Assay Procedure".

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