- Kit Component:
- Cytoplasmic Extraction Reagent (CER) 30ml
Nuclear Extraction Reagent (NER) 5ml
- Kit Detection Method:
- Colorimetric, OD450 nm
- Printable datasheet for OKBB00021
- Reconstitution and Storage:
- Store at 4C for one year.
- Description: This kit supplies a complete set of lysis reagents that enable the separation of nuclear protein and cytoplasmic fractions from cultured cells and tissue. The optimized reagents and protocol allow non-denatured, active proteins to be purified in less than two hours. It is sufficient for extracting 50-100 protein samples, the quantity for each sample is (5-10) x 108 cells (about 50mg).
- Tips Information: