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Catalog No: OKNA00121
Size:96 Wells
Price: $249.00
SKU
OKNA00121
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Cytomegalovirus (CMV) IgG ELISA Kit (Human) (OKNA00121)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Cytomegalovirus (CMV) IgG ELISA Kit (Human) (OKNX00121) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Cytomegalovirus antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for Human IgG is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is qualitatively proportional to the amount of sample anti-Cytomegalovirus IgG captured the in well.
ELISA Kit Reproducibility
MetricSample Typen=CV%
Inter-AssayPositive Serum477.26.2
Intra-AssayPositive Serum122.861.9
ELISA Kit Component
ComponentAmount
Cytomegalovirus IgG Well Plate96 Wells (12 x 8 Well Strips)
IgG Sample Diluent1 x 100 mL
Stop Solution1 x 15 mL
20X Wash Solution1 x 50 mL
Anti-IgG HRP Conjugate1 x 20 mL
TMB Substrate1 x 15 mL
Cytomegalovirus Positive Control1 x 2 mL
Cytomegalovirus Cut-Off Control1 x 3 mL
Cytomegalovirus Negative Control1 x 2 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma
SensitivitySensitivity is determined as the probability of the assay indicating a positive score in samples with the specific analyte present: > 98%
SpecificitySpecificity is determined as the probability of the assay indicating a negative score in samples absent of the specific analyte: > 97.5%
Assay InfoAssay Methodology: Quantitative Reverse Capture Sandwich ELISA
Alias SymbolsCMV
Protein NameCytomegalovirus IgG
Description of TargetCytomegalovirus (CMV) is a member of the herpesvirus group (Betasubfamily, DNA virus of 150-200 nm). These viruses share a characteristic ability to remain dormant within the body over a long period. Initial CMV infection, which may have few symptoms, is always followed by a prolonged, inapparent infection during which the virus resides in cells without causing detectable damage or clinical illness. Severe impairmant of the body´s immune system by medication or disease consistently reactivates the virus from the latent or dormant state. CMV is found universally throughout all geographic locations and socioeconomic groups, and infects between 50% and 85% of adults. CMV infection is more widespread in developing countries and in areas of lower socioeconomic conditions. For the vast majority of people, CMV infection is not a serious problem, but it is to certain high-risk groups: the unborn baby during pregnancy people who work with children immunocompromised persons, such as organ transplant recipients and persons infected with HIV
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