Catalog No: OKIA00176
Size:1 x 96 well plate
Price: $550.00
SKU
OKIA00176
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Cystatin C ELISA Kit (Mouse) (OKIA00176)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration100 Minutes
ELISA Kit PrincipleAviva Systems Biology Cystatin C ELISA Kit (Mouse) (OKIA00176) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Cystatin-C has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Cystatin-C - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cystatin-C captured in the well.
ELISA Kit Range1.25 ng-ml - 40 ng/ml
ELISA Kit Component
ComponentAmount
Anti-MOUSE CYSTATIN C Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
MOUSE CYSTATIN C Calibrator1
Additional InformationIntended Use: The Cystatin C test kit is a highly sensitive two-site Enzyme linked immunoassay (ELISA) for measuring Cystatin C in mouse biological samples.
::REAGENT PREPARATION1. DILUENT CONCENTRATE

e bottle containing 50 ml of a 5X concentrated diluent running buffer.


2. WASH SOLUTION CONCENTRATE

One bottle containing 50 ml of a 20X concentrated wash solution.
3. DETECTION ANTIBODY 100X

One vial containing 150 L of affinity purified anti- Cystatin C antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION

One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION

One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI- Cystatin C ELISA MICRO PLATE

Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti- Cystatin C.
7. MOUSE Cystatin C CALIBRATOR.
One vial containing a lyophilized Mouse Cystatin C calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.146 ng/ml
Predicted Homology Based on Immunogen SequenceMouse
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCST3
Gene Full Namecystatin C
Alias SymbolsCys, CysC
NCBI Gene Id13010
Protein NameCystatin-C
Description of TargetAs an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Uniprot IDP21460
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