Catalog No: OKIA00184
Size:1 x 96 well plate
Price: $500.00
SKU
OKIA00184
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMonkey
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration110 Minutes
ELISA Kit PrincipleAviva Systems Biology Cystatin C ELISA Kit (Monkey) (OKIA00184) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Cystatin-C has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Cystatin-C - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cystatin-C captured in the well.
ELISA Kit Range0.125 ng/ml - 4 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Monkey Cystatin C1 Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Monkey Cystatin C1 Calibrator1
Additional InformationIntended Use: The Cystatin C (CYS) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Cystatin C in biological fluid of Monkey.
::REAGENTS



1. DILUENT CONCENTRATE (Running Buffer)

One bottle containing 50 ml of a 1X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.3. DETECTION ANTIBODY 100X
One vial containing 150 L of affinity purified anti-Monkey CYS antibody conjugated with biotin in a stabilizing buffer.
4. HRP-STREPTAVIDIN 100X
One vial containing 150 L of HRP conjugated streptavidin in a stabilizing buffer.
5. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.36. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.WARNING:
Avoid contact with skin.7. ANTI-MONKEY CYS ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Monkey CYS.8. MONKEY CYS CALIBRATOR
One vial containing Monkey CYS calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.330 ng/ml
Predicted Homology Based on Immunogen SequenceMonkey
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
IntroductionCystatin C is a small cysteine proteinase inhibitor present in body fluids. Studies have shown Cystatin C levels to be directly correlated with the glomular filtration rate.
Alias SymbolsCystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
NCBI Gene Id574313
Protein NameCystatin-C
Description of TargetAs an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Uniprot IDO19092
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