Catalog No: OKCD02486
Size:96 Wells
Price: $700.00
SKU
OKCD02486
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration3 Hours
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-104%94-101%89-102%92-104%
EDTA Plasma (n=5)79-89%93-105%82-91%80-105%
Heparin Plasma (n=5)88-102%94-101%88-102%89-102%
ELISA Kit PrincipleAviva Systems Biology Cyr61 ELISA Kit (Mouse) (OKCD02486) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyr61 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Cyr61 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Cyr61 captured in well.
ELISA Kit Range6.25-400 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)92-10195
EDTA Plasma (n=5)81-10488
Heparin Plasma(n=5)81-9086
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CYR61 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CYR61 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
ELISA Kit Component
ComponentAmount
Anti-CYR61 Microplate96 Wells (12 x 8 Well strips)
CYR61 Lyophilized Standard2
100X Biotinylated CYR61 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity2.36 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). No significant cross-reactivity or interference between Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCYR61
Gene Full Namecysteine rich protein 61
Alias SymbolsCCN, Cyr6, Cyr61, Igfbp10, AI325051
NCBI Gene Id16007
Protein NameProtein CYR61
Description of TargetPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Swissprot IdP18406
Protein Accession #NP_034646.1
Nucleotide Accession #NM_010516.2
Protein Size (# AA)379
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CYR61.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CYR61.
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