Catalog No: OKCD02486
Price: $700.00
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Datasheets/ManualsPrintable datasheet for CYR61 ELISA Kit (Mouse) (OKCD02486)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Serum (n=5)92-104%94-101%89-102%92-104%
EDTA Plasma (n=5)79-89%93-105%82-91%80-105%
Heparin Plasma (n=5)88-102%94-101%88-102%89-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range6.25-400pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)92-10195
EDTA Plasma (n=5)81-10488
Heparin Plasma(n=5)81-9086
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
Anti-CYR61 Microplate96 Wells (12 x 8 Well strips)
CYR61 Lyophilized Standard2
100X Biotinylated CYR61 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity2.36 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). No significant cross-reactivity or interference between Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCcn1
Gene Full Namecellular communication network factor 1
Alias Symbols3CH61;AI325051;CCN;CCN family member 1;Cyr6;Cyr61;cysteine rich protein 61;cysteine-rich angiogenic inducer 61;IBP-10;IGF-binding protein 10;Igfbp10;IGFBP-10;insulin-like growth factor-binding protein 10;intermediate early;protein CYR61.
NCBI Gene Id16007
Protein NameProtein CYR61
Description of TargetPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity). CYR61-mediated gene regulation is dependent on heparin-binding (By similarity). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity).
Uniprot IDP18406
Protein Accession #NP_034646.1
Nucleotide Accession #NM_010516.2
Protein Size (# AA)379
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