Catalog No: OKCD08907
Size:96 Wells
Price: $800.00
SKU
OKCD08907
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CYR61 ELISA Kit (Human) (OKCD08907) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYR61 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYR61 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYR61 captured in the well.
ELISA Kit Range62.5-4000pg/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CYR61. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CYR61 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
CYR61 Microplate96 Wells (12 x 8 Well strips)
CYR61 Lyophilized Standard2
100X CYR61 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 26.1pg/mL
Gene SymbolCYR61
Gene Full Namecysteine rich angiogenic inducer 61
Alias SymbolsGIG1, CYR61, IGFBP10
NCBI Gene Id3491
Protein NameProtein CYR61
Description of TargetCYR61 is a secreted, cysteine-rich, heparin-binding protein encoded by a growth factor-inducible immediate-early gene. Acting as an extracellular, matrix-associated signaling molecule, CYR61 promotes the adhesion of endothelial cells through interaction with integrin and augments growth factor-induced DNA synthesis in the same cell type.
Swissprot IdO00622
Protein Accession #NP_001545.2
Nucleotide Accession #NM_001554.4
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express CYR61.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express CYR61.
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