Catalog No: OKEH00476
Size:96 Wells
Price: $425.00
SKU
OKEH00476
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CYR61 ELISA Kit (Human) : 96 Wells (OKEH00476)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CYR61 ELISA Kit (Human) (OKEH00476) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CYR61 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human CYR61 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CYR61 captured in well.
ELISA Kit Range31.2 - 2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CYR61 Microplate96 Wells (12 x 8 Well strips)
CYR61 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CYR611 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Protein CYR61
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCYR61
Gene Full Namecysteine rich angiogenic inducer 61
Alias SymbolsGIG1, CYR61, IGFBP10
NCBI Gene Id3491
Protein NameProtein CYR61
Description of TargetPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Swissprot IdO00622
Protein Accession #NP_001545.2
Nucleotide Accession #NM_001554.4
Protein Size (# AA)381
Molecular Weight42 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CYR61.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CYR61.
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