Catalog No: OKBB00537
Size:96 Wells
Price: $400.00
Availability: Domestic: within 1 week delivery | International: 1 week
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human CYR61 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CYR61 has been precoated onto 96-well plates. Standards(CHO, A22-D381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CYR61 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CYR61 amount of sample captured in plate.
ELISA Kit Range31.2 pg/ml - 2,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
n 161616242424
Mean (pg/mL)6281145157457010971563
ELISA Kit Component
96-Well plate Pre-coated with Anti-Human CCN1 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human CCN1 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human CCN1 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human CYR61
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: CHO; Immunogen sequence: A22-D381
DescriptionFor quantitative detection of human CYR61 in cell culture supernatants, serum, plasma(heparin, EDTA), saliva, urine and human milk.
Gene SymbolCCN1
Gene Full Namecellular communication network factor 1
Alias Symbols3CH61, CCN 1, CCN family member 1, CCN1, CCN1, CYR 61, Cyr61, CYR61 protein, CYR61 protein precursor, CYR61 protein precursor, CYR61_HUMAN, Cysteine rich angiogenic inducer 61, Cysteine rich heparin binding protein 61, Cysteine-rich angiogenic inducer 61, GIG 1, GIG1, GIG1 protein, IBP-10, IGF-binding protein 10, Igfbp 10, IGFBP-10, IGFBP10, Insulin like growth factor binding protein 10, Insulin-like growth factor-binding protein 10, Protein CYR61, Protein GIG1
NCBI Gene Id3491
Protein NameCCN family member 1
Description of TargetCysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.
Uniprot IDO00622
Protein Accession #NP_001545.2
Nucleotide Accession #NM_001554.4
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