Catalog No: OKEH06493
Size:96T
Price: $525.00
SKU
OKEH06493
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Cyp7b1 ELISA Kit (Mouse) (OKEH06493) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp7b1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cyp7b1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp7b1 captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 102.4%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 6.2% (n = 20)
Mean Inter-Assay Precision - <= 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity39.3 pg/mL
SpecificityNatural and recombinant Mouse 25-hydroxycholesterol 7-alpha-hydroxylase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCyp7b1
Gene Full Namecytochrome P450, family 7, subfamily b, polypeptide 1
Alias Symbols24-hydroxycholesterol 7-alpha-hydroxylase;25/26-hydroxycholesterol 7-alpha-hydroxylase;25-hydroxycholesterol 7-alpha-hydroxylase;3-hydroxysteroid 7-alpha hydroxylase;AW261589;cytochrome P450 7B1;cytochrome P450, 7b1;D3Ertd552;D3Ertd552e;hct-1;hippocampal transcript 1 protein;oxysterol 7-alpha-hydroxylase.
NCBI Gene Id13123
Protein NameQ60991
Description of Target25-hydroxycholesterol 7-alpha-hydroxylase
Uniprot IDhttps://www.uniprot.org/uniprot/Q60991
Protein Accession #NP_001275556
Nucleotide Accession #NM_001288627
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