CYP7A1 ELISA Kit (Rat) (OKEH04424)

Catalog No: OKEH04424

Size:96T

Rat CYP7A1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Cyp7a1 ELISA Kit (Rat) (OKEH04424) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp7a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Cyp7a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp7a1 captured in the well.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Component:
  • Anti-Cyp7a1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Cyp7a1 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Cyp7a1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 3.95 pg/mL
• Specificity: Natural and recombinant Rat Cholesterol 7-alpha-monooxygenase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cyp7a1
• Gene Full Name: cytochrome P450 family 7 subfamily A member 1
• Alias Symbols: 24-hydroxycholesterol 7-alpha-hydroxylase;CHAP;cholesterol 7-alpha-hydroxylase;cholesterol 7-alpha-monooxygenase;CYP7;CYP7S1;CYPVII;cytochrome P450 (cholesterol hydroxylase 7 alpha);cytochrome P450 7A1;cytochrome P450, 7a1;cytochrome P450, family 7, subfamily a, polypeptide 1.
• NCBI Gene Id: 25428
• Protein Name: P18125
• Description of Target: Cholesterol 7-alpha-monooxygenase
• Uniprot ID: https://www.uniprot.org/uniprot/P18125
• Protein Accession #: NP_037074.1
• Nucleotide Accession #: NM_012942.2
• Protein Size (# AA): 503
• Molecular Weight: 57 KDa