CYP7A1 ELISA Kit (Mouse) (OKCD02485)

Catalog No: OKCD02485

Size:96WELLS

Mouse CYP7A1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	81-101%	84-93%	88-99%	86-101%
  EDTA Plasma (n=5)	90-97%	92-99%	85-98%	86-101%
  Heparin Plasma (n=5)	80-98%	80-105%	98-105%	88-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 7A1 (CYP7A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 7A1 (CYP7A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 7A1 (CYP7A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 7A1 (CYP7A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85-104	99
  EDTA Plasma (n=5)	80-101	90
  Heparin Plasma(n=5)	86-99	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 7A1 (CYP7A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 7A1 (CYP7A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CYP7A1 Microplate: 96 Wells (12 x 8 Well strips)
  • CYP7A1 Lyophilized Standard: 2
  • 100X Biotinylated CYP7A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.133 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 7A1 (CYP7A1). No significant cross-reactivity or interference between Cytochrome P450 7A1 (CYP7A1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cyp7a1
• Gene Full Name: cytochrome P450, family 7, subfamily a, polypeptide 1
• Alias Symbols: 24-hydroxycholesterol 7-alpha-hydroxylase;cholesterol 7-alpha-hydroxylase;cholesterol 7-alpha-monooxygenase;CYPVII;CYPVIIc;cytochrome P450 7A1;cytochrome P450, 7a1.
• NCBI Gene Id: 13122
• Protein Name: Cholesterol 7-alpha-monooxygenase
• Description of Target: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
• Uniprot ID: Q64505
• Protein Accession #: NP_031850.2
• Nucleotide Accession #: NM_007824.2
• Protein Size (# AA): 503