CYP4F3 Antibody - N-terminal region (OAAB05240)

Catalog No: OAAB05240

Size:400ul

Western blot analysis of CYP4F3 Antibody (N-term) in K562 cell lysates (35ug/lane). CYP4F3 (arrow) was detected using the purified Pab.

CYP4F3 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC
• Additional Information: Function: Isoform CYP4F3A: Catalyzes the omega-hydroxylation of leukotriene-B(4), a potent chemoattractant for polymorphonuclear leukocytes, it has low activity for arachidonic acid.
  Cellular Location: Endoplasmic reticulum membrane; Single-pass membrane protein Microsome membrane; Single-pass membrane protein.
  Tissue Location: Isoform CYP4F3A is expressed in the polymorphonuclear leukocytes as well as leukocytes and bone marrow. Isoform CYP4F3B is selectively expressed in liver and kidney and is also the predominant CYP4F isoform in trachea and tissues of the gastrointestinal tract.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CYP4F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human CYP4F3.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Application Info: WB: 1:1000
  FC: 1:10~50
• Other Applications Image 1 Data: CYP4F3 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: CYP4F3
• Alias Symbols: CPF3, CYP4F, LTB4H, CYPIVF3
• NCBI Gene Id: 4051
• Protein Name: Leukotriene-B(4) omega-hydroxylase 2
• Description of Target: CYP4F3 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation.
• Uniprot ID: Q08477
• Protein Accession #: NP_000887.2
• Molecular Weight: 60 kDa