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Catalog No: OKCD02045 (Formerly GWB-KBBIZ4)
Size:96WELLS
Price: $750.00
SKU
OKCD02045
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Datasheets/ManualsPrintable datasheet for CYP4F2 ELISA Kit (Human) (OKCD02045)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 4F2 (CYP4F2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 4F2 (CYP4F2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 4F2 (CYP4F2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 4F2 (CYP4F2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 4F2 (CYP4F2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 4F2 (CYP4F2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CYP4F2 Microplate96 Wells (12 x 8 Well Strips)
CYP4F2 Lyophilized Standard2 x 100 ng
100X Biotinylated CYP4F2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.59 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 4F2 (CYP4F2). No significant cross-reactivity or interference between Cytochrome P450 4F2 (CYP4F2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCYP4F2
Gene Full Namecytochrome P450 family 4 subfamily F member 2
Alias Symbols20-HETE synthase;20-hydroxyeicosatetraenoic acid synthase;arachidonic acid omega-hydroxylase;CPF2;CYPIVF2;cytochrome P450 4F2;cytochrome P450, family 4, subfamily F, polypeptide 2;cytochrome P450, subfamily IVF, polypeptide 2;cytochrome P450-LTB-omega;docosahexaenoic acid omega-hydroxylase;leukotriene-B(4) 20-monooxygenase 1;leukotriene-B(4) omega-hydroxylase 1;phylloquinone omega-hydroxylase CYP4F2.
NCBI Gene Id8529
Protein NamePhylloquinone omega-hydroxylase CYP4F2
Description of TargetOmega-hydroxylase that oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids and xenobiotics. Plays a key role in vitamin K catabolism by mediating omega-hydroxylation of vitamin K1 (phylloquinone), and menaquinone-4 (MK-4), a form of vitamin K2. Hydroxylation of phylloquinone and MK-4 probably regulates blood coagulation (PubMed:19297519, PubMed:24138531). Also shows arachidonic acid omega-hydroxylase activity in kidney, by mediating conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), possibly influencing blood pressure control (PubMed:10660572, PubMed:17341693, PubMed:18574070). Also acts as a leukotriene-B(4) omega-hydroxylase by mediating conversion of leukotriene-B(4) (LTB4) to its omega-hydroxylated metabolite 20-hydroxyleukotriene-B(4) (20-OH LTB4) (PubMed:8026587, PubMed:9799565).
Uniprot IDP78329
Protein Accession #NP_001073.3
Nucleotide Accession #NM_001082.4
Protein Size (# AA)520
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