Catalog No: OKCD00720 (Formerly GWB-KBBMU1)
Size:96WELLS
Price: $750.00
SKU
OKCD00720
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Datasheets/ManualsPrintable datasheet for CYP3A7 ELISA Kit (Human) (OKCD00720)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
98-105%96-104%98-105%91-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 3A7 (CYP3A7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 3A7 (CYP3A7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 3A7 (CYP3A7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 3A7 (CYP3A7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
83-9286
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 3A7 (CYP3A7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 3A7 (CYP3A7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CYP3A7 Microplate96 Wells (12 x 8 Well Strips)
CYP3A7 Lyophilized Standard2 x 100 ng
100X Biotinylated CYP3A7 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.57 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 3A7 (CYP3A7). No significant cross-reactivity or interference between Cytochrome P450 3A7 (CYP3A7) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCYP3A7|CYP3A7-CYP3A51P
Gene Full NameCYP3A7-CYP3A51P readthrough|cytochrome P450 family 3 subfamily A member 7
Alias Symbolsaryl hydrocarbon hydroxylase;CP37;CYP3A7;CYP3A7.1L;CYP3A7-3AP1;CYP3A7-CYP3A51P protein;CYP3A7-CYP3AP1;CYP3A7-CYP3AP1 readthrough;CYPIIIA7;cytochrome P450 3A7;cytochrome P450, family 3, subfamily A, polypeptide 7;cytochrome P450, subfamily IIIA, polypeptide 7;cytochrome P450-HFLA;flavoprotein-linked monooxygenase;microsomal monooxygenase;P-450(HFL33);P-450111A7;P450-HFLA;P450HLp2;xenobiotic monooxygenase.
NCBI Gene Id100861540|1551
Protein NameCytochrome P450 3A7
Description of TargetCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Uniprot IDP24462
Protein Accession #NP_000756
Nucleotide Accession #NM_000765.4
Protein Size (# AA)503
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