Catalog No: OCOA08500
Size:20UG
Price: $275.00
SKU
OCOA08500
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CYP3A43 Protein Lysate (OCOA08500)

Datasheets/ManualsPrintable datasheet for CYP3A43 Protein Lysate (OCOA08500)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionCYP3A43 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolCYP3A43
Gene Full Namecytochrome P450 family 3 subfamily A member 43
Alias Symbolscytochrome P450 3A43;cytochrome P450, family 3, subfamily A, polypeptide 43;cytochrome P450, subfamily IIIA, polypeptide 43.
NCBI Gene Id64816
Protein NameNM_022820, NP_073731, Q9HB55
Description of TargetCytochrome P450 3A43, cytochrome P450 3A43 isoform 1 [Homo sapiens], Homo sapiens cytochrome P450 family 3 subfamily A member 43 (CYP3A43), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q9HB55
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_073731
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_022820
Molecular Weight57.6 kDa
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