Catalog No: OKCD08446
Size:96WELLS
Price: $800.00
SKU
OKCD08446
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
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Datasheets/Manuals | Printable datasheet for CYP3A4 ELISA Kit (Human) (OKCD08446) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 3A4 (CYP3A4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 3A4 (CYP3A4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 3A4 (CYP3A4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 3A4 (CYP3A4) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 3A4 (CYP3A4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 3A4 (CYP3A4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.116ng/mL |
Gene Symbol | CYP3A4 |
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Gene Full Name | cytochrome P450 family 3 subfamily A member 4 |
Alias Symbols | 1,4-cineole 2-exo-monooxygenase;1,8-cineole 2-exo-monooxygenase;Albendazole monooxygenase;albendazole monooxygenase (sulfoxide-forming);albendazole sulfoxidase;cholesterol 25-hydroxylase;CP33;CP34;CYP3A;CYP3A3;CYPIIIA3;CYPIIIA4;cytochrome P450 3A3;cytochrome P450 3A4;cytochrome P450 HLp;cytochrome P450 NF-25;cytochrome P450, family 3, subfamily A, polypeptide 4;cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3;cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4;cytochrome P450-PCN1;glucocorticoid-inducible P450;HLP;NF-25;nifedipine oxidase;P450C3;P450-III, steroid inducible;P450PCN1;quinine 3-monooxygenase;taurochenodeoxycholate 6-alpha-hydroxylase;VDDR3. |
NCBI Gene Id | 1576 |
Protein Name | Cytochrome P450 3A4 |
Description of Target | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed:11159812). Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed:21576599). |
Uniprot ID | P08684 |
Protein Accession # | NP_001189784.1 |
Nucleotide Accession # | NM_001202855.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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