CYP3A4 Antibody (OAAF02960)

Catalog No: OAAF02960 (Formerly GWB-ASE135)

Size:100 ug

Western blot analysis of extracts from Jurkat cells, using Cytochrome P450 3A4/5 Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Cytochrome P450 3A4/5.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Cytochrome P450 3A4/5 antibody detects endogenous levels of total Cytochrome P450 3A4/5 protein.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB 1:500~1000
  ELISA 1:10000
  
• Reference: Chen Q., ghua Yu Fang Yi Xue Za Zhi 32:281-284(1998).
  Gellner K., Pharmacogenetics 11:111-121(2001).
  Hsieh K.-P., Drug Metab. Dispos. 29:268-273(2001).

Target Info

• Gene Symbol: CYP3A4|CYP3A5
• Gene Full Name: cytochrome P450 family 3 subfamily A member 4|cytochrome P450 family 3 subfamily A member 5
• Alias Symbols: 1,4-cineole 2-exo-monooxygenase;1,8-cineole 2-exo-monooxygenase;Albendazole monooxygenase;albendazole monooxygenase (sulfoxide-forming);albendazole sulfoxidase;aryl hydrocarbon hydroxylase;cholesterol 25-hydroxylase;CP33;CP34;CP35;CYP3A;CYP3A3;CYPIIIA3;CYPIIIA4;CYPIIIA5;cytochrome P450 3A3;cytochrome P450 3A4;cytochrome P450 3A5;cytochrome P450 HLp;cytochrome P450 HLp2;cytochrome P450 NF-25;cytochrome P450, family 3, subfamily A, polypeptide 4;cytochrome P450, family 3, subfamily A, polypeptide 5;cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3;cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4;cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5;cytochrome P450-PCN1;cytochrome P450-PCN3;flavoprotein-linked monooxygenase;glucocorticoid-inducible P450;HLP;microsomal monooxygenase;NF-25;nifedipine oxidase;P450C3;P450-III, steroid inducible;P450PCN1;P450PCN3;PCN3;quinine 3-monooxygenase;taurochenodeoxycholate 6-alpha-hydroxylase;VDDR3;xenobiotic monooxygenase.
• NCBI Gene Id: 1576|1577
• Protein Name: Cytochrome P450 3A4|Cytochrome P450 3A5
• Description of Target: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.|Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed:11159812). Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed:21576599).
• Uniprot ID: P08684|P20815
• Protein Accession #: NP_000768.1
• Molecular Weight: 57 kDa