CYP3A4 Antibody - middle region (OAAB04841)

Catalog No: OAAB04841

Size:200ul

Western blot analysis of CYP3A4 Antibody (Center) in NCI-H460 cell lysates (35ug/lane). CYP3A4 (arrow) was detected using the purified Pab.

CYP3A4 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

CYP3A4 Antibody (Center) (Cat. OAAB04841)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP3A4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

CYP3A4 Antibody (Center) (Cat. OAAB04841) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
• Additional Information: Cellular Location: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein
  Tissue Location: Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056) According to others, weak levels of expression are measured in some brain locations. Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CYP3A4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the Central region of human CYP3A4.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Sample Type Confirmation:

  CYP3A4 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

• Application Info: WB: 1:500
  WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: CYP3A4
• Alias Symbols: HLP, CP33, CP34, CYP3A, NF-25, VDDR3, CYP3A3, P450C3, CYPIIIA3, CYPIIIA4, P450PCN1
• NCBI Gene Id: 1576
• Protein Name: Cytochrome P450 3A4
• Description of Target: CYP3A4, is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens.
• Uniprot ID: P08684
• Protein Accession #: NP_001189784.1
• Molecular Weight: 57 kDa