CYP2D6 Protein (OPPA00836)

Catalog No: OPPA00836 (Formerly GWB-1B765C)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: LKM-1 is supplied in 50mM Potassium phosphate buffer pH-7.5, 15% glycerol, 1mM DTT and 1% Triton X-100. Physical appearance: Sterile filtered liquid formulation.
• Host: Sf9 Insect Cells
• Application: WB
  Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).
  Product Introduction: Cytochrome P450 2D6 is a member of a complex family of microsomal enzymes (mono-oxygenases) present in the endoplasmic reticulum membrane, which perform detoxification reaction on xenobiotic compounds. Cytochrome P450 2D6 is the molecular target of autoantibodies against the "liver kidney microsomal antigen 1" (LKM 1) which has been classically defined by immunofluorescence microscopy. The presence of these autoantibodies is considered indicative of Autoimmune Hepatitis Type 2; LKM 1 antibodies have also been detected in patients with hepatitis C viral infection. The International Autoimmune Hepatitis Group therefore has subdivided the AIH type 2 into two subgroups: 2a with HCV and 2b without HCV. AIH 2a patients are often over 40 and predominantly male.The use of a purified recombinant Cytochrome P450 2D6 antigen allows the differentiation of autoimmune hepatitis from drug-induced hepatitis where transient autoantibodies to other P450 family members occur which cannot be differentiated by immunofluorescence techniques.
  Product Description: Cytochrome P450 2D6 Human Recombinant (also called liver/kidney microsomal antigen 1) produced in SF9, is a glycosylated, polypeptide chain having a molecular mass of 55,801 Dalton.The LKM1 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20Cfor longer periods of time. Avoid multiple freeze-thaw cycles.
• Purity: Greater than 40% as determined by SDS-PAGE.
• Application Info: Western-Blot with monoclonal anti-LKM-1 antibody and autoimmune hepatitis patient sera.

Target Info

• Gene Symbol: CYP2D6
• Alias Symbols: CPD6, CYP2D, CYP2DL1, CYPIID6, P450C2D, P450DB1, CYP2D7AP, CYP2D7BP, CYP2D7P2, CYP2D8P2, P450-DB1
• NCBI Gene Id: 1565
• Protein Name: Cytochrome P450 2D6
• Description of Target: Recombinant Human Cytochrome P450 2D6
• Uniprot ID: P10635
• Protein Accession #: NP_000097.3
• Protein Size (# AA): Recombinant