Catalog No: OKEH03561
Size:96T
Price: $600.00
SKU
OKEH03561
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CYP2D4 ELISA Kit (Rat) (OKEH03561) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp2d4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cyp2d4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp2d4 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
ComponentAmount
Cyp2d4 Microplate96 Wells (12 x 8 Well strips)
Cyp2d4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Cyp2d4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.182 ng/mL
SpecificityNatural and recombinant Rat Cytochrome P450 2D4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCyp2d4
Gene Full Namecytochrome P450, family 2, subfamily d, polypeptide 4
Alias SymbolsCyp2d18;Cyp2d22;Cyp2d4v1;Cyp2d4v2;Cyp2d6;CYPIID18;CYPIID4;cytochrome P450 2d18;cytochrome P450 2D-29;cytochrome P450 2D-35;cytochrome P450 2D4;cytochrome P450, family 2, subfamily d, polypeptide 22;cytochrome P450, subfamily 2D, polypeptide 6;cytochrome P450, subfamily IID, polypeptide 6;Cytochrome P450, subfamily IID4;cytochrome P450-CMF3;cytochrome P450-DB4;debrisoquine 4-hydroxylase;P450 2D-29/2D-35;P450-CMF3;P450-DB4.
NCBI Gene Id171522
Protein NameQ64680
Description of TargetCytochrome P450 2D4
Uniprot IDhttps://www.uniprot.org/uniprot/Q64680
Protein Accession #NP_612524.1
Nucleotide Accession #NM_138515.2
Protein Size (# AA)500
Molecular Weight57 kDa
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