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Catalog No: OKCD06392
Size:96WELLS
Price: $750.00
SKU
OKCD06392
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Datasheets/ManualsPrintable datasheet for CYP2C19 ELISA Kit (Human) (OKCD06392)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2C19 (CYP2C19). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 2C19 (CYP2C19). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2C19 (CYP2C19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 2C19 (CYP2C19) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 2C19 (CYP2C19) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 2C19 (CYP2C19) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CYP2C19 Microplate96 Wells (12 x 8 Well strips)
CYP2C19 Lyophilized Standard2
100X CYP2C19 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.125ng/mL
Gene SymbolCYP2C19
Gene Full Namecytochrome P450 family 2 subfamily C member 19
Alias Symbols(R)-limonene 6-monooxygenase;(S)-limonene 6-monooxygenase;(S)-limonene 7-monooxygenase;CPCJ;CYP2C;CYPIIC17;CYPIIC19;cytochrome P450 2C19;cytochrome P-450 II C;cytochrome P450, family 2, subfamily C, polypeptide 19;cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 19;cytochrome P450-11A;cytochrome P450-254C;fenbendazole monooxygenase (4'-hydroxylating);flavoprotein-linked monooxygenase;mephenytoin 4'-hydroxylase;mephenytoin 4-hydroxylase;microsomal monooxygenase;P450C2C;P450IIC19;S-mephenytoin 4-hydroxylase;xenobiotic monooxygenase.
NCBI Gene Id1557
Protein NameCytochrome P450 2C19
Description of TargetResponsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, citalopram and imipramine.
Uniprot IDP33261
Protein Accession #NP_000760.1
Nucleotide Accession #NM_000769.2
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