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Catalog No: OKCD01772 (Formerly GWB-KBBWI4)
Size:96WELLS
Price: $675.00
SKU
OKCD01772
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Datasheets/ManualsPrintable datasheet for CYP27A1 ELISA Kit (Human) (OKCD01772)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27A1 (CYP27A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27A1 (CYP27A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27A1 (CYP27A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 27A1 (CYP27A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 27A1 (CYP27A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 27A1 (CYP27A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CYP27A1 Microplate96 Wells (12 x 8 Well Strips)
CYP27A1 Lyophilized Standard2 x 10 ng
100X Biotinylated CYP27A1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 27A1 (CYP27A1). No significant cross-reactivity or interference between Cytochrome P450 27A1 (CYP27A1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCYP27A1
Gene Full Namecytochrome P450 family 27 subfamily A member 1
Alias Symbols5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase;5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase;5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase;cholestanetriol 26-monooxygenase;CP27;CTX;CYP27;cytochrome P450 27;cytochrome P450, family 27, subfamily A, polypeptide 1;cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1;cytochrome P-450C27/25;sterol 26-hydroxylase, mitochondrial;sterol 27-hydroxylase;vitamin D(3) 25-hydroxylase.
NCBI Gene Id1593
Protein NameSterol 26-hydroxylase, mitochondrial
Description of TargetCatalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
Uniprot IDQ02318
Protein Accession #NP_000775.1
Nucleotide Accession #NM_000784.3
Protein Size (# AA)531
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