CYP26B1 Antibody (OAEB00834)

Catalog No: OAEB00834 (Formerly GWB-B94DB0)

Size:100UG

OAEB00834 (4ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Additional Information: Amino Acids: 512 aa
  Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: CYP26B1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Rat: 100%
• Peptide Sequence: C-SQARSEDKDGRFH
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:128000.
  Immunohistochemistry: In paraffin embedded Human Cerebellum shows staining of cell bodies in the Molecular Layer. Recommended concentration, 4-6ug/ml.
  Western Blot: Preliminary experiments gave an approx 85kDa band in human testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_063938.1. The 85kDa band was successfully blocked by incubation with the immunizing peptide.
• Reference: Bowles J, Knight D, Smith C, Wilhelm D, Richman J, Mamiya S, Yashiro K, Chawengsaksophak K, Wilson MJ, Rossant J, Hamada H, Koopman P.
  Retinoid signaling determines germ cell fate in mice.
  Science. 2006 Apr 28;312(5773):596-600. Epub 2006 Mar 30. PMID: 16574820

Target Info

• Gene Symbol: Cyp26b1|CYP26B1
• Gene Full Name: cytochrome P450 family 26 subfamily B member 1|cytochrome P450, family 26, subfamily b, polypeptide 1
• Alias Symbols: CP2;CP26;CYP26A2;cytochrome P450 26B1;cytochrome P450 family 26 subfamily A member 1;cytochrome P450 retinoic acid-inactivating 2;cytochrome P450 retinoid metabolizing protein;cytochrome P450, 26, retinoic acid B1;cytochrome P450, family 26, subfamily B, polypeptide 1;cytochrome P450, subfamily XXVIB, polypeptide 1;cytochrome P450RAI-2;P450R;P450RAI2;P450RAI-2;retinoic acid B1;retinoic acid hydroxylase;retinoic acid-metabolizing cytochrome;RHFCA.
• NCBI Gene Id: 232174|312495|56603
• Protein Name: Cytochrome P450 26B1
• Uniprot ID: Q9NR63
• Protein Accession #: NP_063938.1
• Molecular Weight: 57.5; 49.0