CYP26A1 ELISA Kit (Human) (OKCD01773)

Catalog No: OKCD01773 (Formerly GWB-KBBDF5)

Size:96WELLS

Human CYP26A1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 26A1 (CYP26A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 26A1 (CYP26A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 26A1 (CYP26A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 26A1 (CYP26A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 26A1 (CYP26A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 26A1 (CYP26A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CYP26A1 Microplate: 96 Wells (12 x 8 Well Strips)
  • CYP26A1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CYP26A1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 26A1 (CYP26A1). No significant cross-reactivity or interference between Cytochrome P450 26A1 (CYP26A1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CYP26A1
• Gene Full Name: cytochrome P450 family 26 subfamily A member 1
• Alias Symbols: CP26;CYP26;cytochrome P450 26A1;cytochrome P450 retinoic acid-inactivating 1;cytochrome P450, family 26, subfamily A, polypeptide 1;cytochrome P450, subfamily XXVIA, polypeptide 1;cytochrome P450RAI;hP450RAI;P450, retinoic acid-inactivating, 1;P450RAI;P450RAI1;retinoic acid 4-hydroxylase;retinoic acid-metabolizing cytochrome.
• NCBI Gene Id: 1592
• Protein Name: Cytochrome P450 26A1
• Description of Target: Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18-hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA.
• Uniprot ID: O43174
• Protein Accession #: NP_000774.2
• Nucleotide Accession #: NM_000783.3
• Protein Size (# AA): 497