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Catalog No: OKCD01774 (Formerly GWB-KBBXB7)
Size:96WELLS
Price: $675.00
SKU
OKCD01774
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Datasheets/ManualsPrintable datasheet for CYP24A1 ELISA Kit (Human) (OKCD01774)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 24A1 (CYP24A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 24A1 (CYP24A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 24A1 (CYP24A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 24A1 (CYP24A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 24A1 (CYP24A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 24A1 (CYP24A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CYP24A1 Microplate96 Wells (12 x 8 Well Strips)
CYP24A1 Lyophilized Standard2 x 10 ng
100X Biotinylated CYP24A1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 24A1 (CYP24A1). No significant cross-reactivity or interference between Cytochrome P450 24A1 (CYP24A1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCYP24A1
Gene Full Namecytochrome P450 family 24 subfamily A member 1
Alias Symbols1,25-@dihydroxyvitamin D3 24-hydroxylase;1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial;24-OHase;CP24;CYP24;cytochrome P450 24A1;cytochrome P450, family 24, subfamily A, polypeptide 1;cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase);cytochrome P450-CC24;exo-mitochondrial protein;HCAI;HCINF1;P450-CC24;vitamin D 24-hydroxylase;vitamin D(3) 24-hydroxylase.
NCBI Gene Id1591
Protein Name1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
Description of TargetHas a role in maintaining calcium homeostasis. Catalyzes the adrenodoxin-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.
Uniprot IDQ07973
Protein Accession #NP_000773.2
Nucleotide Accession #NM_000782.4
Protein Size (# AA)514
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